Estimation of Lipid Peroxidation of Spinach Leaf Extract's after Radiation Exposure to Mice Brain

Abstract

This study seeks to investigate the radioprotective efficacy of spinach in mitigating oxidative stress induced by radiation, given its leaves' richness in antioxidants, including proteins, vitamins, ascorbic acid, p-coumaric acid, and carotenoids (lutein, beta-carotene, and zeaxanthin). Healthy male 6-week-old Swiss albino mice were chosen from an inbred colony and kept on regular mouse food and water without restriction. Four groups of mice were used for the tests. There was no treatment given to Group 1 (normal). Group II (drug treated) received a daily oral supplement of spinach extract dissolved in double-distilled water at a level of 1100 mg/kg.b.wt./day for 15 days in a row. For 15 days in a row after being exposed, Group III (experimental) also received oral spinach extract at a rate of 1100 mg/kg.b.wt/day. Supplementing with spinach before irradiation lowers the LPO values, which were significantly elevated by radiation at all examined intervals. Day 30° saw the experimental group's LPO values return to normal, while the control group's LPO values were remained increased by about 12%. It is possible that spinach leaf extract lowers LPO values by squelching free radicals because the amounts of LPO products in the brains of mice given SE supplements activate antioxidant enzymes in the brain. The present study's protective effect of SE on the brain's LPO value suggests that spinach may have some radioprotective properties if consumed regularly. This could be because of the antioxidants' synergistic effects.