EFFECTS OF ARSENIC TRIOXIDE ON BRAIN BIOCHEMISTRY AND BEHAVIOR IN RATTUS NORVEGICUS
DOI:
https://doi.org/10.59436/k4ejjx45Keywords:
Arsenic trioxide, neurotoxicity, free radicals, glutathione peroxidase, total brain proteins, albino rats.Abstract
Toxic exposure to arsenic, which affects almost every organ system, including the brain, is a major health problem for many millions of individuals throughout the globe. This pervasive element may be found in the soil, water and atmosphere as well as creatures, rocks, volcanic emissions, and human activity. For the purpose of this research, arsenic trioxide was used to examine the biochemical effects on the brains of albino rats. Rats were placed into five equal groups, with three albino males in each group. We classified groups I and II as "controls," "acute," and "subacute," with durations ranging from seven days to fourteen days and twenty-one days, respectively. At a dosage of 3.43 mg/Kg b.w.t, rats of II, III, IV and V were given As2O3 orally during 1, 7, 14 and 21 days, respectively. GPx, Na+ -K+ ATPase and Brain total protein concentrations were all shown to be lower after arsenic trioxide poisoning. These biochemical markers were also affected. An arsenic toxicity-induced neurotoxicity and free radical generation in the brain of albino rats led to DNA damage and cell death, according to the findings of this research.
References
Chunxiang, Wu., Xinli, Gu., Yaming, Ge., Zhang. J., Shanxi, J.W. (2006). Effects of high fluoride and arsenic on brain biochemical indices and learning memory in rats. Fluoride, 39 (4) : 274-279.
Das, K.A., Dewanjee, S., Sahu, R., Dua, T.K., Gangopadhyay, M. and Sinha, M.K. (2010). Protective effect of Corchorus olitorius Environmental Toxicology and Pharmacology. 29: 64-69. DOI: https://doi.org/10.1016/j.etap.2009.10.002
Devi, C.B., Kumari, K.K. and Indravathi, G. (2014). Arsenic induced perturbations in cholinergic system and energy metabolism in young and adult rat brain: Reversal effect of vitamin-E. International Journal of Innovative Research in Science, Engineering and Technology. 3 (10): 16840-16849.
Dubey, N.P., Maheshwari, H.S., Jain, S.K. and Rana, A.C. (2008). Studies of As2O3poisoning on protein, RNA and Glycogen of albino rats. Asian J. Exp. Sci. 22(3) : 225 –234.
Herrera, A., Pineda, J. and Antonio, M.T. (2013). Toxic effects of perinatal arsenic exposure on the brain of developing rats and the beneficial role of natural antioxidants. Environmental Toxicology and Pharmacology. 36 : 73 –79. DOI: https://doi.org/10.1016/j.etap.2013.03.018
Jin, Y., Xi, S., Li, X., Lu, C., Li, G., Xu, Y., Qu, C., Niu, Y. and Sun, G. (2006). Arsenic speciation transported through the placenta from mother mice to their newborn pups. Environ. Res., 101: 349-355. DOI: https://doi.org/10.1016/j.envres.2005.11.006
Lowry, O.H., Rosenbrough, N.J., Farr Al, and Ramdall R.J. (1951). Protein measurement with Folin-phenol reagent. J. Biol. Chem., 193: 265-275. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6
Lynn, S., Gurr, J.R., Lai, H.T. and, Jan, K.Y. (2000). NADN Oxidase DNA damage in human vasculas smooth muscle cells. Circulation Research, 86 (5) : 514-519 DOI: https://doi.org/10.1161/01.RES.86.5.514
Muthumani, M. and Prabhu, S.M. (2012). Silibinin potentially protects arsenic-induced oxidative hepatic dysfunction in rats. Toxicology Mechanisms and Methods. 22 (4): 277-288. DOI: https://doi.org/10.3109/15376516.2011.647113
OECD (2001). Acute oral toxicity fixed dose procedure. Test Guideline No. 420, OECD guideline for testing of chemicals, OECD, Paris.
Paglia, D.E. and Valentinem W.N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. lab. Clin. Med., 70: 158-169.
Patlolla, A. K and Tchoubwou, P.B. (2005). Serum Acetyl cholinesterase as a Biomarker of Arsenic induced neurotoxicity in Sprague-Dawley Rats. Int. J. Environ. Res. Public Health, 2 (1) : 80-83 DOI: https://doi.org/10.3390/ijerph2005010080
Prabhu, S.M., Muthumani, M. and Shagirtha, K. (2013). Quercetin potentially attenuates cadmium induced oxidative stress mediated cardiotoxicity and dyslipidemia in rats. European Review for Medical Pharmacological Sciences. 17 : 582 –595.
Ratnaike, R.N. (2003). Acute and chronic arsenic toxicity. Postgrad. Med. J. 79 : 391 –396. DOI: https://doi.org/10.1136/pmj.79.933.391
Rodriguez, V.M., Carrizale, L., Mendoza, M.S., Fajardo, O.R. and Giodano, M. (2002). Effects of sodium arsenite exposure on development and behaviour in rat. Neurotoxicol. Teratol., 24: 743-750.
Rodriguez, V.M., Carrizales, L., Jimenez, C.M.E., Dufour, L. and Giodano, M. (2001). The effect of sodium arsenite exposure on behavioural parameters in rat brain. Res. Bull., 55: 301-308. DOI: https://doi.org/10.1016/S0361-9230(01)00477-4
Rodriguez, V.M., Razo, L.M.D., Limon-Pacheco, J.H., Giordano, M., Sanchez-Pena, L.C., Uribe-Querol, E., Gutierrez-Ospina, G. and Gonsebatt, M.E. (2005). Glutathione reductase inhibition and methylalea arsenic distribution in CD1 Mice Brain and Liver. Toxicological Sciences. 84: 157-166. DOI: https://doi.org/10.1093/toxsci/kfi057
Rodriguez., V.M., Carrizales, L., Mendoza, M.S., Fajardo, O.R. and Giordano M. (2001). Effects of Sodium arsenite exposureon development and behaviour in the rat. Neurotoxicol. Teratol. 24 :743 –750. DOI: https://doi.org/10.1016/S0892-0362(02)00313-6
Vahidnia, A., Vander Voet, G.B. And de wolff, F.A. (2007). Arsenic neurotoxicity –a review. Hum. Exp. Toxicol., 26: 823-832. DOI: https://doi.org/10.1177/0960327107084539
Xi, S., Jin., Y., Lv, X. And Sun, G. (2010). Distribution and speciation of arsenic by transplacental and early life exposure to inorganic arsenic in offspring rats. Biol. Trace Elem. Res., 134: 84-97 DOI: https://doi.org/10.1007/s12011-009-8455-1
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