SYNERGISTIC LARVICIDAL ACTION OF INDIGENOUS PLANT EXTRACTS IN COMBINATION AGAINST AEDES AEGYPTI (DIPTERA: CULICIDAE)
DOI:
https://doi.org/10.59436/jsiane.com/archives2/2/147Keywords:
Aedes aegypti, Argemone mexicana, Prunus persica, Tinospora cordifolia, larvicidal action, LC50, Combination.Abstract
The most effective methods for addressing the problems of insecticide resistance and environmental pollution are expected to be phytochemicals. The current study focuses on evaluating the effectiveness of metabolites from three indigenous plants in combination (Argemone mexicana, Tinospora cordifolia, and Prunus persica) in controlling Aedes aegypti mosquito larvae. The leaf and seed of Argemone mexicana, Prunus persica, and the leaf of Tinospora cordifolia and two solvents (ethanol and petroleum ether) making a total 7 combination groups (A to G) were used in bioassay to determine LC50 and LC90 values. Combination group- A (ethanolic + ethanolic extracts of seed of Prunus persica and Argemone mexicana) LC50 and LC90 values of 70.79 and 169.59 ppm after 24 h post-exposure. Similarly, other combination groups- B, C, D, E, F, and G with LC50 values of 58.88, 74.13, 81.28, 89.12, 61.65, and 57.54 ppm, respectively, and LC90 values of 147.91, 173.78, 229.08, 269.15, 162.18, and 151.35 ppm, respectively, within 24 h. In combination, against the third instar larvae, all the treatments were shown great larvicidal potential (P<0.05). The regression equation showed a dose-dependent mortality, the mortality rate was positively correlated with the concentration. Results showed plant metabolites can be used in combination as eco-friendly insecticides for the control of dengue vectors.
References
Abdullah, F., Ahmad, A., Sabri, M., Sina, I. (2012). Insecticidal properties of stem extracts of Tinospora crispa (Family: Menispermaceae) towards Macrotermes gilvus (Isoptera: Termitidae).
Aragón-Sánchez, M., Serratos, C., Huerta-de la Peña, A., Aragón García, A., Pérez-Torres, B., & Pineda, S. (2020). Effect by Ingestion of Extracts of Argemone mexicana L. on Biological Parameters and Capability of Chrysoperla carnea (Stephens) to Increase in a Laboratory. Southwestern Entomologist. 45:405.
Arokiyaraj, S., Saravanan, M., Udaya Prakash, N., Valan Arasu, M., Vijayakumar, B., & Vincent, S. (2013). Enhanced antibacterial activity of iron oxide magnetic nanoparticles treated with Argemone mexicana L. leaf extract: An in vitro study. Materials Research Bulletin, 48(9): 3323–3327.
Asiry, K.A. (2022). Larvicidal efficacy of leaf extracts of Rhanterium epapposum Oliv. Against dengue fever mosquito vector larvae, Aedes aegypti L., (diptera: Culicidae). Applied Biological Research, 24(1), 87–95.
Aziz, M., Hashan Arif, E. I., Muhammad Dimyati, N. I., Ishak, I. H., Hamdan, R. H., Syazwan, S. A., & Peng, T. L. (2021). Larvicidal effect of Vitex ovata Thunb. (lamiales: Lamiaceae) leaf extract towards Aedes (stegomyia) aegypti (Linnaeus, 1762) (Diptera: Culicidae). Parasitologia, 1(4), 210–217.
Benelli, G. (2015). Research in mosquito control: Current challenges for a brighter future. Parasitology Research, 114(8):2801–2805.
Benelli, G. (2016). Plant-mediated synthesis of nanoparticles: A newer and safer tool against mosquito-borne diseases? Asian Pacific Journal of Tropical Biomedicine, 6(4), 353–354.
Brahmachari, G., Gorai, D., Roy, R. (2013). Argemone mexicana: Chemical and pharmacological aspects. Rev Bras Farmacogn, 23: 559-75.
Carneiro, V. C. S., Lucena, L. B., Figueiró, R., & Victório, C. P. (2020). Larvicidal activity of plants from Myrtaceae against Aedes aegypti L. and Simulium pertinax Kollar (Diptera). Revista da Sociedade Brasileira de Medicina Tropical, 54, e00922020.
Chakraborty, S., Singh, A., & Chandra, G. (2021). Larvicidal activity of different plant parts of asparagus setaceus against dengue vector Aedes aegypti. International Journal of Mosquito Research, 8(5), 01–06.
Christophers, S.R. (1960). Aedes aegypti (L.) The yellow fever mosquito. Its life history, bionomics and structure. Cambridge University Press.
Dass, K., Sujitha, S., & Mariappan, P. (2022). Larvicidal activity of selected medicinal plants against dengue vector Aedes aegypti. International Journal of Mosquito Research, 9(1), 110–113.
Fallatah, S.A. and Khater, E.I. (2010). Potential of medicinal plants in mosquito control. J Egypt Soc Parasitol., 40: 1-26.
Ghosh, A., Chowdhury, N., & Chandra, G. (2012). Plant extracts as potential mosquito larvicides. The Indian journal of medical research, 135(5), 581–598.
Halder, K.M., Ghosh, P., Chandra, G. (2011). Evaluation of target-specific larvicidal activity of the leaf extract of Typhonium trilobatum against Culex quinquefasciatus Say. Asian Pacific Journal of Tropical Biomedicine., 1(2): S199-S203.
Hari, I., & Mathew, N. (2018). Larvicidal activity of selected plant extracts and their combination against the mosquito vectors Culex quinquefasciatus and Aedes aegypti. Environmental Science and Pollution Research, 25(9), 9176–9185.
Jangir, P.K., Prasad, A. (2022). Spatial distribution of insecticide resistance and susceptibility in Aedes aegypti and Aedes albopictus in India. Int J Trop Insect Sci. 42:1019–1044.
Kamaraj, C., Bagavan, A., Rahuman, A.A., Zahir, A.A., Elango, G., Pandiyan, G. (2009). Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitology Research, 104(5):1163–1171.
Kant, R., Shukla, R., Shukla, A. (2018). A Review on Peach (Prunus persica): An Asset of Medicinal Phytochemicals. International Journal for Research in Applied Science and Engineering Technology. 6. 10.22214/ijraset.2018.1342.
Kasiramar, G. (2018). Significant Role of Soxhlet Extraction Process in Phytochemical Research. Mintaje Journal of Pharmaceutical & Medical Sciences, 7:43-47.
Ministry of Health & Family Welfare-Government of India (MOHFW) (2021). National Center for Vector Borne Diseases Control (NCVBDC). Retrieved January 20, 2022,from https://nvbdcp.gov.in/index4.php?lang=1&level=0&linkid=431&lid=3715.
Nancy, A., & Praveena, A. (2018). Argemone mexicana: A boon to medicinal and pharmacological approaches in current scenario. Cardiovascular &Amp; Hematological Agents in Medicinal Chemistry, 15(2): 78–90.
Nobsathian S, Bullangpoti V, Kumrungsee N, Wongsa N, Ruttanakum D. (2018). Larvicidal effect of compounds isolated from Maerua siamensis (Capparaceae) against Aedes aegypti (Diptera: Culicidae) larvae. Chem Biol Technol Agric, 5:1-7
Patil Dipti, R. and Zambare, S.P. (2019). Insecticidal activity of seeds extracts of Argemone mexicana against Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae), Int. J. of. Life Sciences, Special Issue, A13: 102- 105.
Paul, A.V., Raj, S., Vibhuti, A., Pandey, R.P. (2020). Larvicidal Efficacy of Andrographis paniculata and Tinospora cordifolia against Aedes aegypti: A Dengue Vector. Pharmacognosy Research.12(4):352-360.
Ravindran, D. R., Bharathithasan, M., Ramaiah, P., Rasat, M. S. M., Rajendran, D., Srikumar, S., Ishak, I. H., Said, A. R., Ravi, R., & Mohd Amin, M. F. (2020). Chemical composition and larvicidal activity of flower extracts from Clitoria ternatea against Aedes (Diptera: Culicidae). Journal of Chemistry, 2020, 1–9.
Rehman, J.U., Ali, A., Khan, I.A. (2014). Plant-based products: use and development as repellents against mosquitoes: A review. Fitoterapia, 95: 65-74.
Rueda, L. (2004). Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with Dengue Virus Transmission. Zootaxa, 589.
Ruiz-Guerrero, R., Rodriguez-Perez, M.A., Norzagaray-Campos, M. (2015). Toxicity of Mexican native plant extracts against larvae of Aedes aegypti (Diptera: Culicidae). Asian Pac J Trop Biomed, 5(4): 287-291
Saha, S. and Ghosh, S. (2012). Tinospora cordifolia: One plant, many roles. Anc Sci Life. 2012;31(4):151-159.
Sakthivadivel, M., Eapen, A., Dash, A.P. (2012). Evaluation of toxicity of plant extracts against the vector of lymphatic filariasis, Culex quinquefasciatus. Indian J Med Res 135:397-400.
Seo, S.M., Park, I.K. (2012). Larvicidal activity of medicinal plant extracts and lignan identified in Phryma leptostachya var. Asiatica roots against houseflies (Musca domestica L.). Parasitol Res. 110(5):1849-1853.
Sharma, A. K., Tiwari, U., Gaur, M. S., & Tiwari, R. K. (2016). Assessment of malathion and its effects on leukocytes in human blood samples. Journal of Biomedical Research, 30(1): 52–59.
Sharma, K., Saxena, D.B., Gupta, A.K. (2003). Bioefficacy of Tinospora cordifolia and Phyllanthus niruri Plants against Spodoptera litura (Fabricius) and Dysdercus koenigii (Fabricius). Pesticide Research Journal, 15(2):138–142.
Sinha, K., Mishra, N.P., Singh, J., Khanuja, S.P.S. (2004). "Tinospora cordifolia (Guduchi), a reservoir plant for therapeutic applications: A Review". Indian Journal of Traditional Knowledge. 3(3): 257–70.
Sukumar, K., Perich, M. J., & Boobar, L. R. (1991). Botanical derivatives in mosquito control: a review. Journal of the American Mosquito Control Association, 7(2), 210–237.
Tabashnik, B.E. (1994). Evolution of resistance to Bacillus thuringiensis. Annual Review of Entomology., 39:47-79.
Tiwary, M., Naik, S. N., Tewary, D. K., Mittal, P. K., & Yadav, S. (2007). Chemical composition and larvicidal activities of the essential oil of Zanthoxylum armatum DC (Rutaceae) against three mosquito vectors. Journal of vector-borne diseases, 44(3), 198–204.
Vetal, D.S. and Pardeshi, A.B. (2019). Larvicidal potential of Argemone mexicana L. plant extracts against Spodoptera litura fab. Pharma Innovation, 8 (6):698-702.
Warikoo, R., & Kumar, S. (2014). Impact of the Argemone mexicana stem extracts on the reproductive fitness and behavior of adult dengue vector, Aedes aegypti. (Diptera: Culicidae). International Journal of Insect Science, 6, IJIS.S19006.
Wattal, B.L., Joshi, G.C., Das, M. (1981). Role of agriculture insecticides in precipitating vector resistance. Journal of Communicable Diseases., 13:71-73.
World Health Organization (WHO). Dengue and Severe Dengue. (2021). Available online: https://www.who.int/news-room/q-adetail/dengue-and-severe-dengue (accessed on 25 June 2021).
World Health Organization. (2005). Guidelines for laboratory and field testing of mosquito larvicides, Available online: https://apps.who.int/iris/handle/10665/69101
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
