ANTIBIOTIC SUCESPBILITY PATTERN OF PSUEDOMONAS SPECIES ISOLATED FROM WASTE WATER AND SEDIMENTS FROM ABATTOIR IN MAKURDI METROPOLIS

Abstract

To determine the antibiotic susceptibility pattern of Pseudomonas spp isolated from waste water and waste water sediments from abattoir in Makurdi metropolis. The samples of waste water and sediment were collected from drainage point immediately after slaughter slab where the solid parts (sludge) of the sewage was separated with the use of wire mesh to enable free settling sediment. Thus, samples were collected from four different abattoirs, located in North Bank, Wurukum, Modern Market and Wadata area of Makurdi metropolis. All the pseudomonas spp were examined microscopically. The samples were analyzed morphologically, culturally, and further subjected to biochemical tests using standard microbiological practices. The Kirby Bauer disc diffusion method was used for antibiotic susceptibility testing. From the quantification of isolates, the colony count ranged between 35.2x 103Cfu/ml to 2.0x109 Cfu/ml in waste water sediments. Colony count in waste water ranged from 7.2×103 Cfu/ml to 1.6×109 cfu/ml. The isolates showed resistance to Augumentin, Chloramphanicol, Septrin, while Ciprofloxacin, Amoxalin, Streptomycin and perfloxacin were highly susceptible and effective. Adequate treatment of waste water from this abattoir is highly recommended to reduce contamination and spread of infections leading to public health hazards. Furthermore, The butchers, sellers and workers in abattoirs should be educated on the importance of practicing good personal and environmental hygiene so to stop the spread of these organisms.