Identification and In silico analysis of fungal species from plant pathogens
DOI:
https://doi.org/10.59436/swc66e63Keywords:
Asclepias syriaca, Fungal species, Molecular techniques, Phylogenetic analysis, Pongamia pinnata.Abstract
The study aimed to identify, characterize and analyze fungal species associated with two economically significant plant species, Pongamia pinnata and Asclepias syriaca. Through molecular technique, fungal isolates were identified.The study employed DNA sequencing and phylogenetic analysis to classify the fungal isolates accurately. Furthermore, in silico analyses provided insights into the pathogenicity mechanisms and genetic diversity of the identified fungal species. Functional annotation and pathway analysis elucidated metabolic pathways associated with pathogenesis and host interactions. Overall, our integrated approach provides a comprehensive understand of fungal diversity, evolution, and pathogenicity in P.pinnata and A.syriaca. These findings lay the foundation of future research aimed at developing sustainable strategies for disease management and crop improvement in agricultural systems.
References
A.M.M., T., H, J. W., G, D. F., & A, M. B. (2019). The ZtvelB gene is required for vegetative growth and sporulation in the wheat pathogen Zymoseptoria tritici., Front. Microbial., 10. DOI: https://doi.org/10.3389/fmicb.2019.02210
Aamir, S., S.K., & Baghela,A. (2015). A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. Plant Pathol Quor, 5(2), 74-81. DOI: https://doi.org/10.5943/ppq/5/2/6
Adams, R. (2007). Identification of essential oil components by gas chromatography/mass spectrometry. Allured Publishing Corporation.
Bidkar, A., Thakur, N., Bolshette, J., & Gogoi, R. (2014). In-silico Structural and Functional analysis of Hypothethical proteins of Leptospira Interrogans. Biochem Pharmacol. Doi:10.4172/2167-0501.1000136. DOI: https://doi.org/10.4172/2167-0501.1000136
Hait, A. (2022, September 19). GOR method. Retrieved from Wikipedia: http://en.wikipedia.org/wiki/GOR_method
Hariharan, Ganeshamoorthy, A.P., & Kandeeparoopan. (2021). Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review. Frontiers in Cellular and Infection Microbiology, 10. Doi:10.3389/fcimb.2020.600234 DOI: https://doi.org/10.3389/fcimb.2020.600234
John, A.L. (1977). Plant Pathology and Plant Pathogens (3rd ed.). Melbourne
Kovalchuk, A., Driessen, A.J. Phylogenetic analysis of fungal ABC transporters. BMC Genomics 11, 177 (2010). https://doi.org/10.1186/1471-2164-11-177 DOI: https://doi.org/10.1186/1471-2164-11-177
Mridha, M., Jabbar, F., M.K., & et al. (2007). The severity and cause of leaf spot disease of Pongamia pinnata L. and fungicidal control of the pathogen. Institute of Forestry and Environmental Sciences, 18, 236-240. Doi:http://doi.org/10.1007/s11676-007-0048-2 DOI: https://doi.org/10.1007/s11676-007-0048-2
Nameirakpam Nirjanta Devi., J, John Prabakaran., Femina Wahaab. (2012, December). Phytochemical analysis and enzyme analysis of endophytic fungi from Centella asiatica. Asian Pacific Journal of Tropical Biomedicine 2(3):S1280–S1284 DOI:10.1016/S2221-1691(12)60400-6. DOI: https://doi.org/10.1016/S2221-1691(12)60400-6
Petruzzello, M. (2020, February 26). Milkweed. Encyclopedia Britannica. Retrieved from http://www.britannica.com/plant/milkweed
Sambrook, J., & Russell, d. (2001). Molecular Cloning: a laboratory manual. New York: CSHL Press.
Shai, K.N., Dhanyakumara, S.B., Sushma, H.K., Asmatanzeem, B., Halaswamy, H., Shashiraj, K.N., Sreenivasa, N. (2023, May). GC-MS Characterization, Antimicrobial, Antifungal and Anti-Oncogenic Activity of Ethyl Acetate Extract of Aspergillus niger Strain AK-6 isolated from Rhizospheric soil. 45(5), 3733-3756. DOI: https://doi.org/10.3390/cimb45050241
Tamura, K., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular biology and evolution, 28(10), 2731-494 DOI: https://doi.org/10.1093/molbev/msr121
Yadhav, R.D., Jain, S.K., & Verma, A. (2011). Pongamia oinnata: an overview. International Journal of Pharmaceutical Sciences and Research, 2(3), 494.
Zhang HW, Song YC, Tan RX (2006). Biology and Chemistry of endophytes. Nat. Pro.Rep. 23, 2006, 753-771. DOI: https://doi.org/10.1039/b609472b
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