GENETIC DIVERSITY ASSESSMENT IN ENDANGERED MEDICINAL PLANTS USING MOLECULAR MARKERS

Authors

  • Pooja Rai Department of Botany, Government Degree College B.B. Nagar, Bulandshahr, Uttar Pradesh, India

DOI:

https://doi.org/10.59436/jsiane.356.2583-2093

Keywords:

Genetic diversity, endangered medicinal plants, molecular markers, conservation genetics, population structure

Abstract

Endangered medicinal plants are invaluable resources for healthcare, biodiversity, and the economy, yet they face increasing threats from habitat loss, overexploitation, and environmental changes. Preserving their genetic diversity is essential to maintain their adaptability, resilience, and potential for future use in medicine and research. Molecular markers have emerged as powerful tools to accurately assess genetic variation within and among populations of these plants, overcoming the limitations of traditional morphological methods. Techniques such as RAPD, ISSR, AFLP, and SSR provide detailed insights into the genetic structure, levels of polymorphism, and population differentiation. This paper reviews the current methodologies employed in genetic diversity studies of endangered medicinal plants using molecular markers, highlighting case studies where these tools have informed conservation strategies. Understanding the genetic makeup of endangered species enables targeted conservation efforts, including in situ and ex situ management, breeding programs, and restoration initiatives. The integration of molecular marker data into conservation biology thus supports sustainable utilization and long-term survival of these critical plant resources. Finally, we discuss challenges faced in molecular studies and future prospects with emerging genomic technologies to enhance conservation outcomes.

References

Balasubramani, S. P., et al. (2021). Role of medicinal plants in traditional and modern medicine. Journal of Ethnopharmacology, 276, 114184.

Bhatt, R., et al. (2020). Threats and conservation of medicinal plants: A review. Frontiers in Pharmacology, 11, 589691.

Chaudhary, R., et al. (2022). Advances in genomics for conservation of medicinal plants. Plant Biotechnology Reports, 16(2), 183-196.

Frankham, R., et al. (2017). Conservation Genetics. Cambridge University Press.

Gupta, P., & Varshney, R. K. (2020). Molecular markers in plant breeding. Methods in Molecular Biology, 2044, 1-10.

Kumar, S., et al. (2021). SSR-based genetic diversity and population structure in Withania somnifera. Plant Molecular Biology Reporter, 39(1), 65-78.

Patel, M. K., et al. (2019). Genetic diversity analysis and conservation of endangered medicinal plants. Genetic Resources and Crop Evolution, 66, 335-345.

Rao, M. R., & Hodgkin, T. (2020). Morphological versus molecular markers: A critical review. Plant Science Today, 7(1), 1-9.

Sharma, R., et al. (2020). ISSR markers reveal population structure of Nothapodytes nimmoniana. Conservation Genetics, 21, 1057-1067.

Singh, B., & Rawat, M. S. M. (2022). Challenges in genetic assessment of endangered medicinal plants. Biodiversity and Conservation, 31(3), 745-763.

Singh, P., et al. (2022). Genetic erosion in medicinal plants: Causes and conservation. Plants, 11(4), 523.

WHO. (2019). WHO Global Report on Traditional and Complementary Medicine. World Health Organization.

Zhao, Y., et al. (2021). Molecular marker applications in endangered medicinal plants: A review. Molecular Biology Reports, 48, 6451-6465.

Das, S., Singh, A., & Verma, P. (2021). Genetic diversity analysis of Withania somnifera populations using SSR markers. Plant Genetic Resources, 19(4), 318-327.

Doyle, J.J., & Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.

Joshi, V., Sharma, P., & Kumar, A. (2020). Integrating molecular markers and ethnobotanical data for conservation of medicinal plants. Biodiversity and Conservation, 29, 2893–2910.

Kumar, S., Singh, A., & Singh, S. (2016). ISSR-based genetic diversity study of Nothapodytes nimmoniana. Gene, 579(2), 142-148.

Parveen, A., Shanker, R., & Kaul, V. (2011). RAPD-based genetic diversity analysis of endangered Podophyllum hexandrum populations in the Indian Himalayas. Biochemical Systematics and Ecology, 39(2), 253-259.

Patel, R., Sharma, N., & Chatterjee, S. (2022). SNP genotyping in Tinospora cordifolia reveals genetic differentiation and conservation priorities. Scientific Reports, 12, 3456.

Sharma, P., Joshi, V., & Singh, R. (2017). AFLP analysis of Rauvolfia serpentina populations: implications for conservation genetics. Plant Systematics and Evolution, 303, 1021-1033.

Singh, B., & Chaturvedi, S. (2021). Linking molecular diversity with medicinal traits in endangered plants: challenges and prospects. Journal of Ethnopharmacology, 267, 113572.

Singh, S., Kumar, V., & Singh, A. (2019). ISSR marker analysis of genetic diversity in Saussurea costus, an endangered medicinal plant. Molecular Biology Reports, 46(5), 5273–5282.

Verma, D., Sharma, A., & Rana, K. (2022). Genomic approaches for conservation of medicinal plants: future perspectives. Frontiers in Plant Science, 13, 823459.

Downloads

Published

2023-03-10

How to Cite

GENETIC DIVERSITY ASSESSMENT IN ENDANGERED MEDICINAL PLANTS USING MOLECULAR MARKERS. (2023). Journal of Science Innovations and Nature of Earth, 3(1), 89-93. https://doi.org/10.59436/jsiane.356.2583-2093

Similar Articles

1-10 of 89

You may also start an advanced similarity search for this article.