Toxic exposure to arsenic, which affects almost every organ system, including the brain, is a major health problem for many millions of individuals throughout the globe. This pervasive element may be found in the soil, water and atmosphere as well as creatures, rocks, volcanic emissions, and human activity. For the purpose of this research, arsenic trioxide was used to examine the biochemical effects on the brains of albino rats. Rats were placed into five equal groups, with three albino males in each group. We classified groups I and II as "controls," "acute," and "subacute," with durations ranging from seven days to fourteen days and twenty-one days, respectively. At a dosage of 3.43mg/Kg b.w.t, rats of II, III, IV and V were given As2O3 orally during 1, 7, 14 and 21 days, respectively. GPx, Na+-K+ ATPase and Brain total protein concentrations were all shown to be lower after arsenic trioxide poisoning. These biochemical markers were also affected. An arsenic toxicity-induced neurotoxicity and free radical generation in the brain of albino rats led to DNA damage and cell death, according to the findings of this research.